Callus formation ability in cultivated peanuts (<i>Arachis hypogaea</i> L.)

Background: Russia is one of the largest peanut importing countries. At same time, in south country, several zones meet requirements for cultivation. It possible to increase yield existing varieties by using modern biotechnology methods, particular agrobacterial transformation. known from literature data that different genotypes and explants various sources react differently vitro regeneration. Successful regeneration depends on correct protocol, including both type composition media promoting growth induction. Objectives: a technique obtaining regenerants culture. Materials methods: Eight accessions VIR collection origin were used work. Embryonic grown Murashige-Skoog medium supplemented with hormone 2,4-dichlorophenoxyacetic acid (2,4-D). Results conclusions: As result assessing regenerative ability peanuts 2,4-D at concentration 2 g/L, differences callus formation revealed accessions. Those catalog numbers k-793, k-2054 k-2055 did not form organogenic calli, while k-698 k-1987 showed highest percentage embryonic explants.